Entwicklung innovativer Nachweisemethoden für Exosomen und exosomale Bestandteile an schmalbändigen sensorischen Nanostrukturen als Biomarker für die personalisierte Diagnostik bei Krebsfrüherkrankung

Summary This EXODIAGNOS project proposal addresses the development of a novel approach for tumor diagnostics. A new selective, early-stage, non-invasive, express cancer diagnostic procedure utilizing exosomal vesicles and their content recognition by specially designed narrow bandwidth active/passive nano- and microphotonic sensors will be proposed for clinical trials. Our approach is based on three recently suggested novelties. (i) Recognition of exosomal biomarkers – surface and intravesicular proteins and mRNA and DNA nucleic acids – as tumor markers. (ii) Amplitude and phase detection methods using narrow band localized plasmonic resonances and resonances in microcavities, to make a breakthrough in the sensitivity and selectivity of the respective bio and environmental sensors. (iii) Ultra-sensitive detection promised by Active Plasmonic Platform (quantum emitters coupled with the plasmonic nanoresonators and/or microresonators). The narrowband nanoresonators and microphotonic will be functionalized with the complimentary biomolecules which guarantees unambiguous selectivity for the target molecules detection. Special design of the nano and microresonators assures strong amplitude and especially phase response at the binding event. All together allows us to detect the appearance of the tumor cells at early stages, with reasonable detection time of about hours, and high selectivity and reliability. Application of our research in the form of routine clinical diagnostic procedure will give the opportunity to take treatment at the early stages of the disease. Combination of extensive expertise in nano and microphotonics, exosomen and tumor biology (German partners), new active glasses for nanophotonics and sensors and medical devices development (Poland), active nanophotonics (Greece), photonic application in medicine and accessibility to large bank of biomaterials (Russia) assures successful accomplishment of the ambitious goals of this project. Scientific and/or technological objectives The project is a part of the recently established activity which is being developed between several German and foreign research centers. In the frame of this activity we plan to integrate our complementary expertise in areas of optical applications of the passive/active plasmonic nano and microresonators related to the biosensors, exosomal biomarkers, and clinical diagnostic in order to develop new technique for selective, express, early stage, and non-invasive cancer diagnostics.

Ansprechpartner: Nazarenko I
Tel: 270-82100
Email: irina.nazarenko@uniklinik-freiburg.de

Projektbeginn: 01.01.2016
Projektende: 30.04.2018

Nazarenko I

Albert-Ludwigs-Universität Freiburg
Institut für Infektionsprävention und Krankenhaushygiene

Breisacher Str. 115 B
79106 Freiburg

Telefon: 0761-270-82060
Fax: 0761-270-82030
http://www.uniklinik-freiburg.de/iuk/live/index.html

• Bundesministerium für Wirtschaft und Energie BMWi, Bund