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Glucosylierung von Elongationsfaktor eEF1A durch Legionella pneumophila Lgt Glucosyltransferasen

Projektbeschreibung:
Legionella pneumophila is the causative pathogen of Legionnaires' disease, which is characterized by severe pneumonia. From at least 15 serotypes Legionella pneumophila serogroup 1 is most important and responsible for about 80 % of all cases of disease. Legionella bacteria proliferate inside eukaryotic cells. While the natural habitat are amoebae, in humans, Legionella multiply in macrophages, monocytes and epithelial cells. Therefore, they induce a replicative vacuole in host cells. To establish the optimal environment for replication, Legionella translocate ~ 300 bacterial effectors into host cells mainly by means of type-II and -IV secretion systems. Especially the Dot/Icm-type-IV secretion system is cucial for replication of Legionella. Several Legionella effectors target host GTP-binding proteins. For example, RaIF acts as a guanine nucleotide exchange factor (GEF) for Arf1 protein, which is important for vesicle traffic. Rab1 is manipulated by the Legionella effectors DrrA/SidM, LidA, LepB, and SidD, which function as a GEF and/or adenylyltransferase, tehthering factor, GTPase activating protein and deampylase, respectively. Moreover, the Legionella effector AnkX acts as a phosphocholine transferase for Rab1 and Rab35, whereas Lem3 reverses this reaction.

Weitere Informationen: http://portal.uni-freiburg.de/pharmakologie/i
Ansprechpartner: Aktories K
Tel: +49 761 203 5301
Email: klaus.aktories@pharmakol.uni-freiburg.de
Projektlaufzeit:
Projektbeginn: 01.05.2014
Projektende: 30.04.2017
Projektleitung:
Aktories K

Albert-Ludwigs-Universität Freiburg
Institut für Experimentelle und Klinische Pharmakologie und Toxikologie
Abteilung I
Albertstr. 25
79104 Freiburg i. Br.

Telefon: +49-761-2035301
Fax: +49-761-2035311
Email: Klaus.Aktories@pharmakol.uni-freiburg.de
http://www.pharmakologie.uni-freiburg.de/i

Mitarbeiter:
  • Jank T
Kooperationspartner
Einsle O, University of Freiburg Wiese S, University of Freiburg Rospert S, University of Freiburg Rodnina M, MPI Göttingen Belyi Y, Moskau
Finanzierung:

  • DFG, DFG

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