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Glucosylierung von Elongationsfaktor eEF1A durch Legionella pneumophila Lgt GlucosyltransferasenProjektbeschreibung:Legionella pneumophila is the causative pathogen of Legionnaires' disease, which is characterized by severe pneumonia. From at least 15 serotypes Legionella pneumophila serogroup 1 is most important and responsible for about 80 % of all cases of disease. Legionella bacteria proliferate inside eukaryotic cells. While the natural habitat are amoebae, in humans, Legionella multiply in macrophages, monocytes and epithelial cells. Therefore, they induce a replicative vacuole in host cells. To establish the optimal environment for replication, Legionella translocate ~ 300 bacterial effectors into host cells mainly by means of type-II and -IV secretion systems. Especially the Dot/Icm-type-IV secretion system is cucial for replication of Legionella. Several Legionella effectors target host GTP-binding proteins. For example, RaIF acts as a guanine nucleotide exchange factor (GEF) for Arf1 protein, which is important for vesicle traffic. Rab1 is manipulated by the Legionella effectors DrrA/SidM, LidA, LepB, and SidD, which function as a GEF and/or adenylyltransferase, tehthering factor, GTPase activating protein and deampylase, respectively. Moreover, the Legionella effector AnkX acts as a phosphocholine transferase for Rab1 and Rab35, whereas Lem3 reverses this reaction.Projektlaufzeit: Projektbeginn: 01.05.2014Projektleitung: Aktories K Mitarbeiter:
Einsle O, University of Freiburg Wiese S, University of Freiburg Rospert S, University of Freiburg Rodnina M, MPI Göttingen Belyi Y, MoskauFinanzierung:
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