Dr. phil. nat. Roland Nitschke

geb. am 05.07.1957 in Rotenburg/Fulda Diplom Biologie/Biochemie Uni Frankfurt 1982 Promotion Biologie/Biochemie Uni Frankfurt 1986 MPI Biophysik Frankfurt 1986 Physiologisches Institut Uni Freiburg 1986 - 1992 NIH Bethesda 1992 - 1994 Physiologisches Institut Uni Freiburg 1994 - 2000 Biologie I Uni Freiburg 2000 - 2007 Life Imaging Center im ZBSA 2007 - heute

Hauptforschungsgebiete

Zellbiologie, konfokale Mikroskopie, FRET Techniken, Nierenphysiologie, Calcium Signalling

Wichtige Publikationen

1. Ronneberger,O., Liu,K., Rath,M., Ruebeta,D., Mueller,T., Skibbe,H., Drayer,B., Schmidt,T., Filippi,A., Nitschke,R., Brox,T., Burkhardt,H., and Driever,W. ViBE-Z: a framework for 3D virtual colocalization analysis in zebrafish larval brains. Nature Methods (2012), doi: 10.1038/nmeth.2076. [Epub ahead of print] 2. Tay,T.L., Ronneberger,O., Ryu,S., Nitschke,R., Driever,W. Analysis of the Catecholaminergic projectome reveals integration of ascending and descending dopaminergic systems in Zebrafish, Nat. Comm. 2 (2011), 171 doi:10.1038 /ncomms1171. 3. Lienkamp,S., Ganner,A., Boehlke,C., Schmidt,T., Arnold,S., Schäfer,T., Romaker,D., Schuler,J., Hoff,S., Powelske,C., Eifler,A., Krönig,C., Bullerkotte,A., Nitschke,R., Kuehn,E.W., Kim,E., Burkhardt,H., Brox,T., Ronneberger,O., Gloy,J., Walz,G. Inversin relays Frizzled-8 signals to promote proximal pronephros development, Proc.Natl.Acad.Sci.U.S.A, 107(47) (2010), 20388-20393. 4. Boehlke,C., Kotsis,F., Patel,V., Voelker,H., Bredt,S., Beyer,T., Müller,K., Herbst,M., Hornung,M., Doerken,M., Köttgen,M., Nitschke,R., Igarashi,P., Walz,G. Kuehn,E.W. Primary cilia regulate mTORC1 activity and cell size through Lkb1, Nat.Cell.Bio., 11 (2010), 1115-1122. 5. Resch-Genger,U., Grabolle,M., Nitschke,R. ,Nann,T. Nanocrystals and nanoparticles vs. molecular fluorescent labels as reporters for bioanalysis and the life sciences - A critical comparison, Advanced Fluorescence Reporters in Chemistry and Biology II in Springer Series on Fluorescence Methods and Applications Chapt. 9, Springer (2010), 3-40. 6. Boehlke,C., Bashkurov,M., Buescher,A., John,A.K., Krick,T., Nitschke,R., Walz,G. Kuehn,E.W. Differential role of Rabs in ciliary trafficking: Rab23 regulates Smoothened levels, J.Cell Science, 123 (2010), 1460-1467. 7. Emmenlauer,M., Ronneberger,O., Ponti,A., Schwarb,P., Griffa,A., Filippi,A., Nitschke,R., Driever,W., Burkhardt,H. XuvTools: Free, fast and reliable stitching of large 3D datasets. J.Microsc., 233 (2009), 42-60. 8. Wegierski,T., Steffl,D., Kopp,C., Tauber,R., Buchholz,B., Nitschke,R., Kuehn,E.W., Walz,G., Köttgen,M. TRPP2 channels regulate apoptosis via the Ca2+ concentration in the endoplasmic reticulum. EMBO J., 28(5) (2009), 490-499. 9. Ditengou,F.A., Teale,W.D., Kochersperger,P., Flittner,K.A., Kneuper,I., van der Graaff,E., Nziengui,H., Pinosa,F., Li,X., Nitschke,R., Laux,T., Palme,K. Mechanical induction of lateral root initiation in Arabidopsis thaliana. Proc.Natl.Acad.Sci.U.S.A, 105 (2008), 18818-18823. 10. Kotsis,F., Nitschke,R., Doerken,M., Walz,G., Kuehn,E.W. Flow modulates centriole movements in tubular epithelial cells. Pflugers Arch., 456 (2008), 1025-1035. 11. Hoffmann,K., Resch-Genger,U., Nitschke,R. Comparability of fluorescence microscopy data and need for instrument characterization of spectral scanning microscopes in Standardization and Quality Assurance in Fluorescence Measurements II, Springer Series on Fluorescence Chapt. 6, Springer (2008) 89-116. 12. Resch-Genger,U., Grabolle,M., Cavaliere-Jaricot,S., Nitschke,R., Nann,T. Quantum dots versus organic dyes as fluorescent labels. Nat.Methods, 5 (2008), 763-775. 13. Kotsis,F., Nitschke,R., Boehlke,C., Bashkurov,M., Walz,G., Kuehn,E.W. Ciliary calcium signaling is modulated by kidney injury molecule-1 (Kim1). Pflugers Arch., 453(6) (2007), 819-829. 14. Kuppig,S., Nitschke,R. A fusion tag enabling optical marking and tracking of proteins and cells by FRET-acceptor photobleaching. J.Microsc., 222 (2006), 15-21. 15. Resch-Genger,U., Hoffmann,K., Nietfeld,W., Engel,A., Neukammer,J., Nitschke,R., Ebert,B., Macdonald,R. How to improve quality assurance in fluorometry: Fluorescence-inherent sources of error and suited fluorescence standards. J.Fluoresc., 15 (2005), 337-362. 16. Bauer,D., Viczian,A., Kircher,S., Nobis,T., Nitschke,R., Kunkel,T., Panigrahi,K.C., Adam,E., Fejes,E., Schafer,E., Nagy,F. Constitutive photomorphogenesis 1 and multiple photoreceptors control degradation of phytochrome interacting factor 3, a transcription factor required for light signaling in Arabidopsis. Plant Cell, 16 (2004), 1433-1445. 17. Nitschke,R., Henger,A., Ricken,C., Müller,V., Köttgen,M., Bek,M., Pavenstädt,H. Acetycholine increases the free intracellular calcium concentration in podocytes in intact rat glomeruli via muscarinic M5 receptors. J.Am.Soc.Nephrol., 12 (2001), 678-687. 18. Nitschke,R., Henger,A., Ricken,S., Gloy,J., Müller,V., Greger,R., Pavenstädt,H. Angiotensin II increases intracellular calcium activity in podocytes of the intact glomerulus. Kidney Int., 57 (2000), 41-49. 19. Ricken,S., Leipziger,J., Greger,R., Nitschke,R. Simultaneous measurements of cytosolic and mitochondrial Ca2+ transients in HT29 cells. J.Biol.Chem., 273(52) (1998), 34961-34969. 20. Nitschke,R., Wilhelm,S., Borlinghaus,R., Bindels,R.J.M., Leipziger,J., Greger,R. A modified confocal laser scanning microscope allows fast ultraviolet ratio imaging of intracellular Ca2+ activity using fura-2. Pflugers Arch., 433 (1997), 653-663. 21. Nitschke,R., Ricken,S., Leipziger,J., Greger,R. Coupling of InsP3 induced cytosolic and mitochondrial Ca2+ changes in HT29 cells. Nova Acta Leopold, 302 (1997), 163 22. Nitschke,R., Benning,N., Ricken,S., Leipziger,J., Fischer,K.-G., Greger,R. Effect of intracellular pH on agonist-induced [Ca2+]i transients in HT29 cells. Pflugers Arch., 434 (1997), 466-474. 23. Nitschke,R., Riedel,A., Ricken,S., Leipziger,J., Benning,N., Fischer,K.-G., Greger,R. The effect of intracellular pH on cytosolic Ca2+ in HT29 cells. Pflugers Arch., 433 (1996), 98-108. 24. Nitschke,R., Spring,K.R. Electro-optical wavelength selection enables confocal ratio imaging at low light levels. J.Micro.Soc.Am., 1 (1995), 1-11. 25. Nitschke,R., Leipziger,J., Greger,R. Agonist induced intracellular Ca2+ transients in HT29 cells. Pflugers Arch., 423 (1993), 519-526. 26. Nitschke,R., Leipziger,J., Greger,R. Intracellular Ca2+ transients in HT29 cells induced by hypotonic cell swelling. Pflugers Arch., 423 (1993), 274-279. 27. Nitschke,R., Fröbe,U., Greger,R. Antidiuretic hormone acts via V1 receptors on intracellular calcium in the isolated perfused rabbit cortical thick ascending limb. Pflugers Arch., 417 (1991), 622-632.

Kontaktaufnahme

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Email: Roland.Nitschke@biologie.uni-freiburg.de
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