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Molekulare Analyse der Phytohormonwirkung – Gezielter Knockout von Mitgliedern der Phytohormon-Signalstrecken

Projektbeschreibung:
Auxin, cytokinin and abscisic acid (ABA) induce specific developmental steps in juvenile moss tissues. Here, developmental decisions rely on the differentiation of single cells and gene functions can be studied by reverse genetics via targeted gene knockouts. These tools were used to analyse phytohormone influences on early moss development and the integration of environmental cues via phytohormone signal transduction pathways. Currently, we evaluate the consequences of phytohormones on early gene expression events via micro array analysis. Our aim is to fully unravel the complex regulatory signal transduction networks underlying phytohormone-driven developmental decisions of single plant cells.

Weitere Informationen: http://www.plant-biotech.net
Ansprechpartner: Prof. Dr. Ralf Reski
Tel: 0761 203 6969
Email: ralf.reski@biologie.uni-freiburg.de
Projektlaufzeit:
Projektbeginn: 01.06.1999
Projektende: (unbegrenzt)
Projektleitung:
Reski R, Decker EL, Frank W
Stellvertretung: Dr. Wolfgang Frank
Albert-Ludwigs-Universität Freiburg

Finanzierung:

  • Schwerpunktprogramm, DFG

Schlagworte:

    bryophyte, phytohormones, cell cycle, computational biology, homologous recombination, cell differentiation

Projektbezogene Publikationen:

  • Frank W, Decker EL, Reski R: Molecular tools to study Physcomitrella patens. Plant Biology, 2005; 7 (3): 220-227.
  • Frank W, Ratnadewi D, Reski R: Physcomitrella patens is highly tolerant against drought, salt and osmotic stress. Planta, 2005; 220 (3): 384-394.
  • Bierfreund NM, Tintelnot S, Reski R, Decker EL: Loss of GH3 function does not affect phytochrome-mediated development in a moss, Physcomitrella patens. J Plant Physiol, 2004; 161 (7): 823-835.
  • Kroemer K, Reski R, Frank W: Abiotic stress response in the moss Physcomitrella patens: evidence for an evolutionary alteration in signaling pathways in land plants. Plant Cell Rep, 2004; 22 (11): 864-870.
  • Lorenz S, Tintelnot S, Reski R, Decker EL: Cyclin D-knockout uncouples developmental progression from sugar availability. Plant Mol Biol, 2003; 53 (1-2): 227-236.

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