Anaerober Stoffwechsel von Aromaten

Aromaten bilden nach den Kohlenhydraten die zweithäufigste organische Stoffklasse in der Natur. Sie sind deshalb wichtige Substrate für das Wachstum von Bakterien und Pilzen. Um den stabilen aromatischen Ring spalten zu können, braucht es gewöhnlich molekularen Sauerstoff. Aromaten werden aber auch von Bakterien oxidiert, die in Sauerstoff-freien Bereichen der Erde leben. Der anaerobe Abbau muss offenkundig ganz anderen Prinzipien folgen. Da das Grundwasser frei von Sauerstoff ist, haben die Ergebnisse auch Bedeutung für die biologische Beseitigung von Umweltgiften. Einige der Prozesse könnten biotechnologisch interessant sein, wie beispielsweise die Carboxylierung von Phenolen

Weitere Informationen: http://www.biologie.uni-freiburg.de/data/bio2/fuchs/anaerobeStoffwechsel.htm
Ansprechpartner: Fuchs Georg
Tel: 0761-203-2649
Email: georg.fuchs@biologie.uni-freiburg.de

Projektbeginn: 2001
Projektende: (unbegrenzt)

Fuchs G

Albert-Ludwigs-Universität Freiburg

• Barragan MJL, Carmona M, Zamarro MT, Thiele B, Boll M, Fuchs G, Garcia JL, Diaz E: The bzd gene cluster coding for the anaerobic benzoate catabolism in Azoarcus sp. CIB. J. Bacteriol, 2004; 186:5762-5774: 5762-5774.
• Schühle K, Fuchs G: Phenylphosphate carboxylase. A new C-C lyase involved in anaerobic phenol metabolism J. Bacteriol, 2004; 186: 4556-4567.
• Schühle K, Gescher J, Feil U, Paul M, Jahn M, Schägger H, Fuchs G: Benzoate-coenzyme A ligase from Thauera aromatica. One enzyme acting in anaerobic and aerobic benzoate pathways J. Bacteriol, 2003; 185: 4920-4929.
• Mechichi T, Stackebrandt E, Gad'on N, Fuchs G: Phylogenetic and metabolic diversity of bacteria degrading aromatic compounds under denitrifying conditions, and description of Thauera phenylacetica sp. nov., Thauera aminoaromatica sp. nov., and Azoarcus buckelii sp. nov. Arch. Microbiol, 2002; 178: 26-35.
• Boll M, Fuchs G, Meier C, Trautwein A, Lowe DJ: EPR and Mossbauer studies of benzoyl-CoA reductase. J Biol Chem, 2000; 275 (41): 31857-31868. : http://www.jbc.org/cgi/content/abstract/275/41/31857
• Rhee SK, Fuchs G: Phenylacetyl-CoA:acceptor oxidoreductase, a membrane-bound molybdenum-iron-sulfur enzyme involved in anaerobic metabolism of phenylalanine in the denitrifying bacterium Thauera aromatica. Eur J Biochem, 1999; 262 (2): 507-515.
• Breese K, Boll M, Alt-Morbe J, Schagger H, Fuchs G: Genes coding for the benzoyl-CoA pathway of anaerobic aromatic metabolism in the bacterium Thauera aromatica. Eur J Biochem, 1998; 256 (1): 148-154.
• Hirsch W, Schagger H, Fuchs G: Phenylglyoxylate:NAD+ oxidoreductase (CoA benzoylating), a new enzyme of anaerobic phenylalanine metabolism in the denitrifying bacterium Azoarcus evansii. Eur J Biochem, 1998; 251 (3): 907-915.
• Laempe D, Eisenreich W, Bacher A, Fuchs G: Cyclohexa-1,5-diene-1-carbonyl-CoA hydratase [corrected], an enzyme involved in anaerobic metabolism of benzoyl-CoA in the denitrifying bacterium Thauera aromatica. Eur J Biochem, 1998; 255 (3): 618-627.
• Boll M, Albracht SS, Fuchs G: Benzoyl-CoA reductase (dearomatizing), a key enzyme of anaerobic aromatic metabolism. A study of adenosinetriphosphatase activity, ATP stoichiometry of the reaction and EPR properties of the enzyme. Eur J Biochem, 1997; 244 (3): 840-851.
• Heider J, Fuchs G: Anaerobic metabolism of aromatic compounds. Eur J Biochem, 1997; 243 (3): 577-596.
• Heider J, Fuchs G: Microbial anaerobic aromatic metabolism Anaerobe, 1997; 3: 1-22.
• Schneider S, Mohamed ME, Fuchs G: Anaerobic metabolism of L-phenylalanine via benzoyl-CoA in the denitrifying bacterium Thauera aromatica. Arch Microbiol, 1997; 168 (4): 310-320.
• Bonting CF, Fuchs G: Anaerobic metabolism of 2-hydroxybenzoic acid (salicylic acid) by a denitrifying bacterium. Arch Microbiol, 1996; 165 (6): 402-408.
• Biegert T, Fuchs G: Anaerobic oxidation of toluene (analogues) to benzoate (analogues) by whole cells and by cell extracts of a denitrifying Thauera sp. Arch. Microbiol, 1995; 163: 407-417.
• Boll M, Fuchs G: Benzoyl-coenzyme A reductase (dearomatizing), a key enzyme of anaerobic aromatic metabolism. ATP dependence of the reaction, purification and some properties of the enzyme from Thauera aromatica strain K172. Eur J Biochem, 1995; 234 (3): 921-933.
• Bonting CFC, Schneider S, Schmidtberg G, Fuchs G: Anaerobic degradration of m-cresol via methyl oxidation to 3-hydroxybenzoate by a denitrifying bacterium Arch. Microbiol, 1995; 164: 63-69.
• Fuchs G, Mohamed M, Altenschmidt U, Koch J, Lack A, Brackmann R, Lochmeyer R, Oswald B: Biochemistry of anaerobic biodegradation of aromatic compounds In: Kluwer Academic Publishers (Hrsg.): Ratledge C (ed) Biochemistry of microbial degradation Dordrecht, 1994; 513-553.
• Lack A, Fuchs G: Evidence that phenol phosphorylation to phenylphosphate is the first step in anaerobic phenol metabolism in a denitrifying Pseudomonas sp. Arch Microbiol, 1994; 161 (2): 132-139.
• Biegert T, Altenschmidt U, Eckerskorn C, Fuchs G: Enzymes of anaerobic metabolism of phenolic compounds. 4-Hydroxybenzoate-CoA ligase from a denitrifying Pseudomonas species. Eur J Biochem, 1993; 213 (1): 555-561.
• Brackmann R, Fuchs G: Enzymes of anaerobic metabolism of phenolic compounds. 4-Hydroxybenzoyl-CoA reductase (dehydroxylating) from a denitrifying Pseudomonas species. Eur J Biochem, 1993; 213 (1): 563-571.
• Hartel U, Eckel E, Koch J, Fuchs G, Linder D, Buckel W: Purification of glutaryl-CoA dehydrogenase from Pseudomonas sp., an enzyme involved in the anaerobic degradation of benzoate. Arch Microbiol, 1993; 159 (2): 174-181.
• Koch J, Eisenreich W, Bacher A, Fuchs G: Products of enzymatic reduction of benzoyl-CoA, a key reaction in anaerobic aromatic metabolism. Eur J Biochem, 1993; 211 (3): 649-661.