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HIV inhibition by synthetic humate, mechanism of inhibition
Description of the project:
: A complex of compounds (HS-1500) similar to natural humic acids was synthesized by oxidation of hydroquinone at high pH. HS-1500 inhibited the HIV-1 infection of MT-2 cells with an IC50 of 50-300 ng/ml, and a mean cell toxicity of about 600 µg/ml. Treatment of free and cell attached virus with HS-1500 irreversibly reduced its infectivity, whereas the susceptibility of target cells was not impaired by treatment prior to infection. HS-1500 bound to the HIV envelope protein gp120 and interfered with the CD4-induced proteolytic cleavage of the V3-loop of virion gp120. Furthermore, binding of specific antibodies 9284 and 9305 to the V3 loop was inhibited, whereas binding of soluble CD4 to gp120 on virus and infected cells was not affected. In conclusion, our data suggest, that the synthetic humic acid analogue inhibits the infectivity of HIV particles by interference with a V3 loop mediated step of virus entry.
Additional information: www.ukl.uni-freiburg.de/mikrobio/homeeng.htm
Phone: (0761) 203-6588
Email: schf@ukl.uni-freiburg.de
Runtime:
Start of project: 11.06.1994
End of project: 31.12.1997
Project Management:
Albert-Ludwigs-University Freiburg
Schneider Josef
Institut für Medizinische Mikrobiologie und Hygiene
Abteilung Virologie
Hermann-Herder-Strasse 11
79104 Freiburg
Germany
Phone: +49 761 203 6534
Fax: +49 761 203 6626
Email: haller.office@uniklinik-freiburg.de
http://www.virologie.uniklinik-freiburg.de
Actual Research Report
Financing:
- Land Baden-Württemberg, Klinikum
Keywords:
project-related publications:
- Schneider J., Weis R., Maenner C., Kary B., Werner A.: Inhibition of HIV-1 in cell culture by synthetic humate analogues derived from hydroquinone: Mechanism of inhibition. Virology, 1996; 218: 389-395.
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