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 Research Report for the year 2005

Institut für Biochemie und Molekularbiologie

Zentrum für Biochemie und Molekulare Zellforschung (ZBMZ)

Hermann-Herder-Str. 7
79104 Freiburg i. Br.
Tel: +49(0)761/203-5225 Fax: +49(0)761/203-5253
Email inge.friesse@biochemie.uni-freiburg.de
http://www.biochemie.uni-freiburg.de/


Scientific Employees

  • Prof. Dr. R. Brandsch
  • Prof. Dr. K. Decker
  • Prof. Dr. M. Müller
  • Prof. Dr. N. Pfanner
  • Prof. Dr. Sabine Rospert
  • Dr. Dirk Schneider
  • Prof. Dr. M. Tropschug
  • Dipl. Biol. Sandra Angelini
  • Dipl. Biol. R. Antonaea
  • Dipl. Biol. Dorothea Becker
  • Dipl. Biol. Natalie Bolender
  • Diana Boy
  • Dipl. Ing. (FH) K. Brandner
  • Dr. J. Brix
  • cand. med. Jonas Bürk
  • Dr. A. Chacinska
  • Dipl. Biol. C. Chiribau
  • Charlotte Conz
  • Dipl. Biol. Jan Dudek
  • Dipl. Biol. G. Eisner
  • Dr. Silke Fiedler
  • Dipl. Biol. Carmen Finger
  • Dipl. Biol. A. Frazier
  • Dr. Kip Gabriel
  • Dipl. Biol. Petra Ganas
  • Dipl. Biol. Sven Gathmann
  • Dr. Natalia Gebert
  • Dipl. Biol. Eva Holzapfel
  • Dipl. Biol. Dana Huta
  • Prof. Dr. H. G. Koch
  • Dipl. Biol. V. Kozjak
  • Dipl. Biol. Martin Krayl
  • Dipl. Biol. Carmen Kulajta
  • Dipl. Biochem. Stephan Kutik
  • Dipl. Biol. Claudia Leidhold
  • Dipl. Biol. Claus-Philip Maier
  • Prof. Dr. Chris Meisinger
  • Dipl. Molmed. David Mick
  • Dipl. Biol. Marius Mihasan
  • Dr. M. Moser
  • Dipl. Biol. Stefan Oellerer
  • Dipl. Biol. Hendrik Otto
  • Dipl. Biol. Sascha Panahandeh
  • Dipl. Biochem. Kristin Peisker
  • Dipl. Biol. Alexander Prodöhl
  • Dipl. Biol. Magdalena Rakwalska
  • Dipl. Biol. Uta Raue
  • Dr. P. Rehling
  • Dipl. Biol. Michael Rissler
  • Dipl. Biol. Eva Rupprecht
  • Dipl. Biol. Paula Sachelaru
  • Dipl. Biol. Luiza Sanjuan Szklarz
  • Dipl. Biol Grit Sprie
  • Dr. Diana Stojanovski
  • Dipl. Biol. Michal Strüber
  • Dr. Rebecca Taylor
  • Dr. T.-A. Tran-Thi
  • Dipl. Biol. Dirk Tremmel
  • Dr. Martin van der Laan
  • Dr. Thomas Volkmer
  • Dipl. Biol. Ulrike von Plehwe
  • Dr. W. Voos
  • Dipl. Biol. Karina Wagner
  • Dipl. Biol Dorothea Walter
  • Dr. N. Wiedemann
  • Dipl. Biol. Yanfeng Li
  • Dipl. Biol. Tamara Major
  • Dipl. Biol. Dusanka Milenkovic
  • Dipl. Biol. Sylvia Pfannschmidt
  • Dipl. Biol. Birgit von Janowsky
  • Dipl. Biol. Daniel Braun
Entries in "Who is Who"

Main Research

  • Prof. N. Pfanner: Mechanismus der Proteinsortierung und Proteinfaltung in der Zelle, hier insbesondere Targeting und Assemblierung von mitochondrialen Proteinen
  • Dr. Dirk Schneider: Faltung von Membranproteinen und die Sortierung von Proteinen über Membranen in Cyanobakterien
  • Prof. Dr. Sabine Rospert: Identifikation und Charakterisierung von Proteinen, die mit dem Ribosom assoziieren und Untersuchung ihrer Wechselwirkungen mit neusynthetisierten Polypeptidketten.
  • Prof. M. Müller: Mechanismen des Proteintransports durch Membranen und der Proteininsertion in Membranen
  • Prof. M. Tropschug: Zelluläre Funktion von Peptidyl-Prolyl-cis-trans-Isomerasen
  • Prof. R. Brandsch: Biogenese kovalent flavinylierter Enzyme und ihre Rolle als Autoantigen beim Menschen
  • Prof. (em.) K. Decker: 3D-Strukturen bakterieller Flavoproteine

Financing

  • DFG Projekt "In vitro Tat-Transport von Bakterien"
  • EU-Projekt QLK3-CT-1999-00917 "Exploitation of a novel Sec-independent secretion pathway for protein production"
  • FEBS Fellowship
  • Fonds der Chem. Industrie "Chemierelevante Grundlagenforschung im Bereich Bioinformation"
  • Forschungskommision MÜL25/01
  • Gottfried Wilhelm Leibniz Research Award für Prof. N. Pfanner
  • Graduiertenkolleg "Biochemie der Enzyme" GK 439
  • Max Planck Research Award for International Cooperation für Prof. N. Pfanner
  • Teilprojekt A 10 Sonderforschungsbereich 388 "Zelluläre Funktionen dynamischer Proteinwechselwirkungen"
  • Teilprojekt A 11 Sonderforschungsbereich 388 "Zelluläre Funktionen dynamischer Proteinwechselwirkungen"
  • Teilprojekt A2 Sonderforschungsbereich 388 "Zelluläre Funktionen dynamischer Proteinwechselwirkungen"
  • Teilprojekt A8 Sonderforschungsbereich 388 "Zelluläre Funktionen dynamischer Proteinwechselwirkungen"

Scientific and Research Projects



  • Double-Arginin dependent protein export (Tat)
    • Project Manager: Prof. Dr. M. Müller
    • Start/End of project: since 1999
    • Project Details
  • Function of Molybdopterin Cofactor Biosynthetic Enzyme MoeA
    • Project Manager: Prof. Dr. R. Brandsch
    • Start/End of project: since 1998
    • Project Details
  • Functions of the bacterial and archaeal SRPs (signal recognition particle)
    • Project Manager: Dr. H.-G. Koch, Prof. Dr. M. Müller
    • Start/End of project: since 1998
    • Project Details
  • Functions of mitochondrial chaperones, in particular the role of Hsp70 and cochaperones as import motor for preproteins
    • Project Manager: Dr. W. Voos, Dr. C. Meisinger
    • Start/End of project: since 1998
    • Project Details
  • Transfer of hydrophilic and hydrophobic preproteins to the inner mitochondrial membrane and the role of a membrane potential
    • Project Manager: Dr. P. Rehling, Dr. K. Truscott
    • Start/End of project: since 1998
    • Project Details
  • Scientific publications

    Journal Articles:
    • Angelini S, Deitermann S, Koch HG: FtsY, the bacterial signal-recognition particle receptor, interacts functionally and physically with the SecYEG translocon. EMBO Rep, 2005; 6 (5): 476-481
    • Becker L, Bannwarth M, Meisinger C, Hill K, Model K, Krimmer T, Casadio R, Truscott KN, Schulz GE, Pfanner N, Wagner R: Preprotein Translocase of the Outer Mitochondrial Membrane: Reconstituted Tom40 Forms a Characteristic TOM Pore. J Mol Biol, 2005; 353 (5): 1011-1020
    • Blaudeck N, Kreutzenbeck P, Muller M, Sprenger GA, Freudl R: Isolation and characterization of bifunctional Escherichia coli TatA mutant proteins that allow efficient tat-dependent protein translocation in the absence of TatB. J Biol Chem, 2005; 280 (5): 3426-3432
    • Brandner K, Mick DU, Frazier AE, Taylor RD, Meisinger C, Rehling P: Taz1, an outer mitochondrial membrane protein, affects stability and assembly of inner membrane protein complexes: implications for Barth Syndrome. Mol Biol Cell, 2005; 16 (11): 5202-5214
    • Brandner K, Rehling P, Truscott KN: The carboxyl-terminal third of the dicarboxylate carrier is crucial for productive association with the inner membrane twin-pore translocase. J Biol Chem, 2005; 280 (7): 6215-6221
    • Chacinska A, Lind M, Frazier AE, Dudek J, Meisinger C, Geissler A, Sickmann A, Meyer HE, Truscott KN, Guiard B, Pfanner N, Rehling P: Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17. Cell, 2005; 120 (6): 817-829
    • Chiribau CB, Sandu C, Igloi GL, Brandsch R: Characterization of PmfR, the transcriptional activator of the pAO1-borne J Bacteriol, 2005; 187 (9): 3062-3070
    • Deitermann S, Sprie GS, Koch HG: A dual function for SecA in the assembly of single-spanning membrane proteins in. J Biol Chem, 2005; 280: 39077-3907785
    • Huang P, Gautschi M, Walter W, Rospert S, Craig EA: The Hsp70 Ssz1 modulates the function of the ribosome-associated J-protein Zuo1. Nat Struct Mol Biol, 2005; 12 (6): 497-504
    • Kulajta C, Thumfart JO, Haid S, Daldal F, Koch HG: Multi-step Assembly Pathway of the cbb(3)-type Cytochrome c Oxidase Complex. J Mol Biol, 2005
    • Kuruma Y, Nishiyama K, Shimizu Y, Muller M, Ueda T: Development of a minimal cell-free translation system for the synthesis of presecretory and integral membrane proteins. Biotechnol Progr, 2005; 21 (4): 1243-1251
    • Otto H, Conz C, Maier P, Wolfle T, Suzuki CK, Jeno P, Rucknagel P, Stahl J, Rospert S: The chaperones MPP11 and Hsp70L1 form the mammalian ribosome-associated complex. P Natl Acad Sci Usa, 2005; 102 (29): 10064-10069
    • Prodohl A, Volkmer T, Finger C, Schneider D: Defining the structural basis for assembly of a transmembrane cytochrome. J Mol Biol, 2005; 350 (4): 744-756
    • Reif S, Randelj O, Domanska G, Dian EA, Krimmer T, Motz C, Rassow J: Conserved mechanism of Oxa1 insertion into the mitochondrial inner membrane. J Mol Biol, 2005; 354 (3): 520-528
    • Rissler M, Wiedemann N, Pfannschmidt S, Gabriel K, Guiard B, Pfanner N, Chacinska A: The Essential Mitochondrial Protein Erv1 Cooperates with Mia40 in Biogenesis of Intermembrane Space Proteins. J Mol Biol, 2005; 353: 485-492
    • Sachelaru P, Schiltz E, Igloi GL, Brandsch R: An alpha/beta-Fold C-C Bond Hydrolase Is Involved in a Central Step of Nicotine Catabolism by Arthrobacter nicotinovorans. J Bacteriol, 2005; 187 (24): 8516-8519
    • Sandu C, Chiribau B-C, Sachelaru P, Brandsch R: Plasmids for nicotine-dependent and independent gene expression in Arthrobacter nicotinovorans and other Arthrobacter species. Appl Environ Microb, 2005; 71: 8920-8924
    • Sanjuan Szklarz LK, Guiard B, Rissler M, Wiedemann N, Kozjak V, van der Laan M, Lohaus C, Marcus K, Meyer HE, Chacinska A, Pfanner N, Meisinger C: Inactivation of the mitochondrial heat shock protein zim17 leads to aggregation of matrix hsp70s followed by pleiotropic effects on morphology and protein biogenesis. J Mol Biol, 2005; 351 (1): 206-218
    • van der Laan M, Chacinska A, Lind M, Perschil I, Sickmann A, Meyer HE, Guiard B, Meisinger C, Pfanner N, Rehling P: Pam17 is required for architecture and translocation activity of the mitochondrial protein import motor. Mol Cell Biol, 2005; 25 (17): 7449-7458
    • von Janowsky B, Knapp K, Major T, Krayl M, Guiard B, Voos W: Structural properties of substrate proteins determine their proteolysis by the mitochondrial AAA+ protease Pim1. Biol Chem, 2005; 386 (12): 1307-1317
    • Wenk SO, Schneider D, Boronowsky U, Jager C, Klughammer C, de Weerd FL, van Roon H, Vermaas WF, Dekker JP, Rogner M: Functional implications of pigments bound to a cyanobacterial cytochrome b6f complex. Febs Lett, 2005; 272 (2): 582-592
    • Wittig A, Moss RL, Stecher-Rasmussen F, Appelman K, Rassow J, Roca A, Sauerwein W: Neutron activation of patients following boron neutron capture therapy of brain tumors at the high flux reactor (HFR) Petten (EORTC Trials 11961 and 11011). Strahlenther Onkol, 2005; 181 (12): 774-782
    • Zahedi RP, Meisinger C, Sickmann A: Two-dimensional benzyldimethyl-n-hexadecylammonium chloride/SDS-PAGE for membrane proteomics. Proteomics, 2005; 5 (14): 3581-3588
    • Zara V, Ferramosca A, Papatheodorou P, Palmieri F, Rassow J: Import of rat mitochondrial citrate carrier (CIC) at increasing salt concentrations promotes presequence binding to import receptor Tom20 and inhibits membrane translocation. J Cell Sci, 2005; 118 (Pt 17): 3985-3995
    Reviews:
    • Muller M: Twin-arginine-specific protein export in Escherichia coli. Res Microbiol, 2005; 156 (2): 131-136
    • Muller M, Klosgen RB: The Tat pathway in bacteria and chloroplasts (review). Mol Membr Biol, 2005; 22 (1-2): 113-121
    • Rospert S, Rakwalska M, Dubaquie Y: Polypeptide chain termination and stop codon readthrough on eukaryotic ribosomes. Rev Physiol Bioch P, 2005; 155: 1-30
    • Schneider D, Schmidt CL: Multiple Rieske proteins in prokaryotes: where and why? Bba-bioenergetics, 2005; 1710 (1): 1-12
    Book Chapters:
    • Rospert S, Gautschi M, Rakwalska M, Raue U: Ribosome-bound proteins acting on newly synthesized polypeptide chains. , 429-458. In: Protein Folding Handbook. , Vol. II. In Buchner, J. and Kiefhaber, T. (eds.) Wiley-VCH, 2005; 429-458.
    • Voos W, Pfanner N: The chaperone system of mitochondria. In: Protein Folding Handbook, Part II (Buchner, J., and Kiefhaber, T., eds.) Wiley-VCH, Weinheim, 2005; 1020-1046.

    Special Scientific Activities

    • PD Dr. P. Rehling: Jahrespreis der wissenschaftlichen Gesellschaft, 01.11.2004. .
    • Peter Rehling: Young Investigator Award of the GBM and Schering Stiftung, 01.10.2005. .
    • Prof. Dr. N. Pfanner: Mitglied der "Deutsche Akademie der Naturforscher Leopoldina", 01.01.2000. .
    • Prof. Dr. N. Pfanner: Mitglied der EMBO (European Molecular Biology Organisation), 01.01.1994. .
    • Prof. Dr. N. Pfanner: Editor: Reviews of Physiology, Biochemistry & Pharmacology (1999-); Biocimica et Biophysica Acta - Molec. Cell Research (2000-); Editorial/ Advisory Board: European Journal of Biochemistry (1996-); Molecular Membrane Biology (1996-); Current Biology (1998-); Journal of Biological Chemistry (1998-); EMBO Journal (1999-), Biological Cemistry (2000-); EMBO Reports (2000-), seit 2000.
    • Prof. Dr. N. Pfanner: Gottfried Wilhelm Leibniz Research Award, 01.02.2004 bis 31.01.2007.
    • Prof. Dr. Nikolaus Pfanner: Max-Planck-Forschungspreis für Biowissenschaften und Medizin, 03.12.2002. .

    Scientific Dissertations

    Dissertations
    • Dusanka Milenkovic: Identification and functional analysis of new components of the sorting and assembly machinery (SAM) of the yeast mitochondrial outer membrane (Supervisor Pof. N. Pfanner), 2005.
    • Jan Dudek: Charakterisierung neuer Komponenten des mitochondiralen Importmotors für Vorstufenproteine (Supervisor Prof. N. Pfanner, Secondary Supervisor Prof. M. Müller), 2005.
    • Magdalena Rakwalska: The function of the ribosome associated chaperones RAC and Ssb1/2p in translational fidelity in Saccharomyces cerevisiae. (Supervisor Prof. S. Rospert, Secondary Supervisor Prof. M. Müller), 2005.
    • Sandra Angelini: GTP-regulated membrane binding of FtsY, the bacterial SRP receptor (Supervisor Prof. Dr. Matthias Müller, Secondary Supervisor Prof. Dr. Peter Graumann), 2005.
    • Vera Kozjak: folgt, 2005.
    • Yanfeng Li: In vitro characterization of mitochondrial Hsp70 and its partner proteins during protein import in yeast Saccharomyces cerevisiae (Supervisor Prof. N. Pfanner), 2005.